Tan Haidong

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Kirsten Heimann

Sandrine UTTENWEILER

Institute de pharmacology and structural Biology (IPBS)

University of Waikato

Ian McDonald

Shamsudeen Dandare

Queen's University Belfast

Jay Gulledge

Timothy Stemmler

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Purification and Biochemical Characterization of Soluble Methane Monooxygenase Hydroxylase from Methylosinus trichosporium IMV 3011

Bioscience, Biotechnology, and Biochemistry, 2007

Methane monooxygenase hydroxylase was purified by chromatography and characterized by electrophor... more Methane monooxygenase hydroxylase was purified by chromatography and characterized by electrophoresis and spectroscopy. The molecular mass of hydroxylase was 201.3 KDa as determined by gel filtration, whereas the total molecular mass was 234 KDa as judged by SDS-PAGE. Structure study indicated that the enzyme is a homodimer structure, consisting of three subunits, designated , , and , with molecular masses of 58 KDa, 36 KDa, and 23 KDa respectively. IEF analysis indicated that the enzyme has a pI of 5.2. The UV-Vis spectrum of hydroxylase revealed an absorption peak near 281 nm and a weak shoulder peak around 395 nm-420 nm, and a fluorescence spectrum revealed an emission peak at 341.3 nm. Circular dichroism measurement indicated that hydroxylase mainly consists of-helical regions. Finally, phylogenetic analysis indicated that this strain is very close to Methylosinus trichosporium OB3b.

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5-ヒドロキシメチルフルフラールの生物触媒酸化の研究進展【JST・京大機械翻訳】

5-hydroxymethylfurfural(HMF)is an important bio-based platform chemical. Oxidation of HMF to fura... more 5-hydroxymethylfurfural(HMF)is an important bio-based platform chemical. Oxidation of HMF to furan chemicals is a crucial developing orientation of biomass conversion. In recent years，with the advantages of high selectivity，mild reaction conditions， and environment friendly，the biocatalytic oxidation of HMF has attracted extensive attentions. In this paper，we reviewed the discovery of biotransformation process，microbial metabolism，whole-cell biotransformation and the enzymatic catalytic oxidation of HMF. Finally，we provided the development prospect of bio-catalytic oxidation of HMF in the future，aiming at providing the theoretical foundation for the green and efficient conversion of HMF to furan bulk chemicals.

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Purification and Biochemical Characterization of Soluble Methane Monooxygenase Hydroxylase from Methylosinus trichosporium IMV 3011

Http Dx Doi Org 10 1271 Bbb 60402, May 22, 2014

Purification and Biochemical Characterization of Soluble Methane Monooxygenase Hydroxylase from Methylosinus trichosporium IMV 3011

Bioscience, Biotechnology, and Biochemistry, 2007

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5-ヒドロキシメチルフルフラールの生物触媒酸化の研究進展【JST・京大機械翻訳】

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Purification and Biochemical Characterization of Soluble Methane Monooxygenase Hydroxylase from Methylosinus trichosporium IMV 3011

Http Dx Doi Org 10 1271 Bbb 60402, May 22, 2014

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