Saleem Shaikh

Followers

Following

Public Views

Cedric Mercier

APHM

Mahmoud M Sheha

Assiut University

ABDALLA ELBASHIR

University of Khartoum

nura hidayah

Universitas setia budi surakarta

Teresa Garrigues

University of Valencia

Manuel Alos

Alaa Khedr

King AbdulAziz University (KAU) Jeddah, Saudi Arabia

Riin Rebane

Kazuhiro Imai

Musashino University

Maarja-liisa Oldekop

Interests

Uploads

Papers by Saleem Shaikh

A simple and rapid high-performance liquid chromatographic method for the determination of bisoprolol fumarate and hydrochlorothiazide in a tablet dosage form

A simple and sensitive reversed-phase liquid chromatographic method has been developed for the de... more A simple and sensitive reversed-phase liquid chromatographic method has been developed for the determination of amikacin by derivatization. The method is based on the pre-column derivatization of amikacin with 9-fluorenylmethyl chloroformate (FMOC-Cl). Isepamicin was used as the internal standard. The derivatization reaction proceeds in aqueous solution at room temperature with a borate buffer of pH 7.3. The formation of the corresponding derivative of amikacin is instantaneous and it is stable for more than 48 h. Detection was performed by fluorescence. Several factors influencing the derivatization reaction yields were studied and optimized. The system offered the following analytical parameters: limit of detection (LOD) of 90 ng mL -1 (3s), linear correlation coefficient of 0.9998 and linear range response from 0.45 to 21.60 mg·mL -1 . The precision of the method was < 6%. As a preliminary application, the method has been successfully applied to the amikacin determination in parenteral pharmaceutical formulations.

Download

Development and validation of a selective online dissolution method for rosiglitazone maleate

Journal of chromatographic science, 2007

A new specific, accurate, precise, and reproducible selective online dissolution method for rosig... more A new specific, accurate, precise, and reproducible selective online dissolution method for rosiglitazone maleate is developed and validated for the dissolution of rosiglitazone maleate in pharmaceutical formulations. The rationale of the method is based on the direct measurement of the absorbance of the analyte in the buffer medium at 242 nm using buffer as blank. Dissolution is achieved on a dissolution test apparatus consisting of photo diode array spectrophotometer, peristaltic pump, and temperature controller, using 0.01N HCl and 0.05M potassium chloride as the dissolution medium. The proposed method is developed, optimized, and validated in terms of linearity, reproducibility, accuracy, and selectivity for the dissolution of rosiglitazone maleate in pharmaceutical formulations. The method is found to be linear in the range of 1 to 14 microg/mL of rosiglitazone maleate with a correlation coefficient of 0.999. The dissolution studies of rosiglitazone maleate tablets obtained by ...

Download

Determination of sodium hyaluronate in pharmaceutical formulations by HPLC–UV

Journal of Pharmaceutical Analysis, 2013

A liquid chromatography (HPLC) method with UV detection was developed for determination of sodium... more A liquid chromatography (HPLC) method with UV detection was developed for determination of sodium hyaluronate in pharmaceutical formulation. Sodium hyaluronate is a polymer of disaccharides, composed of D-glucuronic acid and D-N-acetylglucosamine, linked via alternating β-1, 4 and β-1, 3 glycosidic bonds. Being a polymer compound it lacks a UV absorbing chromophore. In the absence of a UV absorbing chromophore and highly polar nature of compound, the analysis becomes a major challenge. To overcome these problems a novel method for the determination of sodium hyaluronate was developed and validated based on size exclusion liquid chromatography (SEC) with UV detection. An isocratic mobile phase consisting of buffer 0.05 M potassium dihydrogen phosphate, pH adjusted to 7.0 using potassium hydroxide (10%) was used. Chromatography was carried out at 25 1C on a BioSep SEC S2000, 300 mm Â 7.8 mm column. The detection was carried out using variable wavelength UV-vis detector set at 205 nm. The compounds were eluted isocratically at a steady flow rate of 1.0 mL/min. Sodium hyaluronate retention time was about 4.9 min with an asymmetry factor of 1.93. A calibration curve was obtained from 1 to 38 g/mL (r40.9998). Within-day % RSD was 1.0 and between-day % RSD was 1.10. Specificity/selectivity experiments revealed the absence of interference from excipients, recovery from spiked samples for sodium hyaluronate was 99-102. The developed method was applied to the determination of sodium hyaluronate in pharmaceutical drug substance and product.

Download

A New Simple and Rapid Method for the Determination of Sodium Hyaluronate in Active Pharmaceutical Ingredient and Ophthalmic Formulations by DP5 Photorode

World Journal of Analytical Chemistry, 2014

A new, simple, rapid and reliable turbidimetric routine method for the determination of sodium hy... more A new, simple, rapid and reliable turbidimetric routine method for the determination of sodium hyaluronate in active pharmaceutical ingredient and ophthalmic solution is described by using DP5 Phototrode. The turbidity of an aqueous ophthalmic sample solution was measured using a DP5 Phototrode™ for photometric indicated titration. Near the equivalence point, a precipitate between titrant and analyte is formed, and the solution becomes turbid. The method involves measurement of the equivalence point at minimum light transmission through the sample, containing cationic quaternary ammonium compound, cetylpyridinium chloride (CPC) solution as a dispersing agent, is measured at 520 nm. The results obtained for in-house prepared formulation and other marketed ophthalmic solution are compared with those obtained by the published HPLC method. A calibration curve was obtained from 0.08 to 0.122 mg mL-1 (r > 0.9998). Within-day % RSD was 1.08 and between-day % RSD was 1.10. Specificity/ selectivity experiments revealed the absence of interference from excipients, recovery from spiked samples for sodium hyaluronate was 98.3-100.9%. The developed method was applied to the determination of sodium hyaluronate in pharmaceutical drug substance and drug product.

Download

A simple and rapid high-performance liquid chromatographic method for determining tobramycin in pharmaceutical formulations by direct UV detection

Pharmaceutical Methods, 2011

A Simple RP-HPLC Method for the Simultaneous Quantitation of Chlorocresol, Mometasone Furoate, and Fusidic Acid in Creams

Journal of Chromatographic Science, 2009

A simple, specific, and precise high-performance liquid chromatographic method is developed and v... more A simple, specific, and precise high-performance liquid chromatographic method is developed and validated for the simultaneous determination of chlorocresol (CC), mometasone furoate (MF), and fusidic acid (FA) in a cream formulation. The isocratic mobile phase consists of 1.5% w/v aqueous ammonium acetate buffer-acetonitrile, 55:45 (v/v) of pH 3.8. The column contains octylsilyl chemically bonded to porous silica particle (Symmetry C8, 150x3.9 mm, 5 microm). The detection is carried out using variable wavelength UV-vis detector set at 240 nm. The solutions are chromatographed at a steady flow rate of 1.0 mL/min. The current method separates CC, MF, and FA in less than 8 min with good resolution and peak shapes, minimal tailing, and with retention factors between approximately 1 and 5. Linearity range and percent recoveries for CC, MF, and FA are 10-30, 10-30, and 200-600 microg/mL; and 100.31%, 100.38%, and 100.34%, respectively. The method is validated according to International Conference on Harmonization guidelines and proven to be suitable for stability testing, content uniformity testing, and quality control of these compounds in pharmaceutical preparations.