Papers by Dr.S. Irudaya Monisha
Recently, development of reliable experimental protocols for synthesis of metal nanoparticles wit... more Recently, development of reliable experimental protocols for synthesis of metal nanoparticles with desired morphologies and sizes has become a major focus of researchers. Green synthesis of metal nanoparticles using plant extracts emerged as a nontoxic and ecofriendly method for synthesis of metal nanoparticles. In this study biosynthesis of stable copper nanoparticles were done using aqueous leaf extract of Vitex negundo leaf from 3mm copper sulphate solution. Synthesized nanoparticles were characterized under UV-Vis spectroscopy at the range of 400 nm to 800 nm at varying intervals of time. The peak at 254nm revealed the presence of CuNPs. It was observed that the Vitex negundo leaf extract can reduce copper ions into copper nanoparticles within 10 to 15 min of reaction time. These biosynthesized Cu nanoparticles were characterized with the help of X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and SEM techniques. GC-MS analysis revealed that the leaf extr...
Biosciences, Biotechnology Research Asia, 2018
Plants are made up of chemicals of differing nature produced by metabolism. These phytochemicals ... more Plants are made up of chemicals of differing nature produced by metabolism. These phytochemicals show varied biological activity. The present work was aimed to identify such active principle present in the stem bark of Manilkara hexandra (Roxb.)Dubard. Proximate, fluorescence, histochemical, mineral analysis was carried out for the plant material. From the preliminary analysis, the flavonoid rich extract was identified which was then subjected to using chromatographic methods for compound separation. Characterization of the separated compound was done by UV, FT-IR, and GC-MS studies. 7,9- DI-TERT-BUTYL-1-OXASPIRO[4.5]DECA-6,9-DIENE-2,8-DIONE was identified by GC-MS analysis. Antibacterial activity was carried and it shows high zone of inhibition, when concentration increases from 30 to 150 μg/ml. The separated flavanoid in future could be used to study its wide pharmacological applications as a lead compound in drug delivery.
International Journal of Pharmacy and Pharmaceutical Sciences, 2017
Objective: Natural products from medicinal plants, either as isolated compounds or as standardize... more Objective: Natural products from medicinal plants, either as isolated compounds or as standardized plant extracts exhibit promising source of medicinal activity against various diseases. The aim of the present work was to make an attempt of isolation of bioactive principle and characterization of the isolated compound, from the medicinal plant Melia dubaiMethods: The extraction was done by a cold percolation method and the compound was separated and isolated by chromatography technique such as a thin layer chromatography (TLC), column chromatography and high-performance liquid chromatography (HPLC). The isolated compound was crystallized and the structural characterization of the isolated compound was made using UV-Visible, FT-IR, 1H-NMR, GC-MS and MS techniques which confirmed the structure of the isolated compound.Results: The separated and isolated compound was characterized by both physical and spectral methods like Ultraviolet-Visible spectroscopy (UV-Visible), Fourier transfor...
International Journal of Applied Pharmaceutics, 2019
Objective: In the present study, the isolated natural polymer from the stem of Manilkara hexandra... more Objective: In the present study, the isolated natural polymer from the stem of Manilkara hexandra and their physiochemical parameters were investigated. It is further involved in hypoglycemic studies. Methods: The gum exudates were screened for phytochemicals, physicochemically analyzed for solubility, pH, total ash, moisture content, acid insoluble ash, water soluble ash, sulfated ash, flow property, and it is characterized by Fourier Transform Infrared analysis (FT-IR), Scanning Electron Microscope (SEM), X-ray diffraction analysis (XRD), Nuclear Magnetic Resonance (NMR) studies (13Carbon and 1Proton) and it was examined in vitro studies by hypoglycaemic activity. Results: The isolated gum extracted from the stem of Manilkara hexandra (Roxb.) Dubard ash values were found to be low. The gum is found to be hygroscopic in nature due to its high moisture content (0.9131±0.03). Fourier Transform Infrared (FT-IR) spectra show relevant functional groups for gum, which is further confirme...
Nanotechnology play a major role in research and this nanotechnology are classified into various ... more Nanotechnology play a major role in research and this nanotechnology are classified into various types of nanomaterials. During the most recent decades, the green synthesis of metal nanoparticles has huge significance of both academic research and innovation. In drug plant play a major role. The phytochemical present in the plant has efficient in curing and healing human diseases. This traditional method is merged with metal. This mixture of traditional and new method formulates green synthesis. This method has high impact on social needs for the curing of human ills. The phytochemical present in the plant extract act as capping and stabilizing agent with metal to form metal nanoparticles. Here, biological method is carried which is better than physical and chemical method. This method is eco-friendly, nontoxic and easy to synthesize. Their application is extended in various fields and has different topology.
International Journal of Scientific & Technology Research, 2019
The plant poly phenols are present adequately which has high impact on human drugs. Flavan3ol con... more The plant poly phenols are present adequately which has high impact on human drugs. Flavan3ol consist in the stem of Manilkara hexandra and it has rich medicinal value like anti-oxidant. The physical and chemical properties were investigated from the stem of Manilkara hexandra. Proximate, histochemical, fluorescence, mineral analysis were examined by using standard procedures. The flavan-3-ol was isolated using chromatographics method such as coloumn chromatography and thin layer chromatography. Then it was characterized by UV, FT-IR, LC-Ms, NMR (1H & 13 C) and it is further extended to in-vitro anti-oxidant (DPPH method) potential. The flavanoid was identified by preliminary investigation after then flavan-3-ol was isolated and it gives good response for anti-oxidant activity. The isolated catechin has a unique biological behaviours, and act as a metal chelating agent. So it can be used for further research from this plant.
Journal of Pharmacognosy and Phytochemistry, 2017
The indigenous plant of Manilkara hexandra belongs to Sapotaceae family. It is also called in tam... more The indigenous plant of Manilkara hexandra belongs to Sapotaceae family. It is also called in tamil “Kanuppalai”. The sacred plant is widely observed in the religious temple of South India because of it consists of latex. The investigation is carried on phytochemical screening analysis, quantitative and yield percentage of leaf, stem, and bark of Manilkara hexandra. The dried powder was screened with petroleum ether, hexane, chloroform, ethyl acetae, ethanol, methanol and double distilled water by cold extraction method. The secondary metabolites were estimated with standard procedures. The yield percentage was calculated. The qualitative report shows the presence of phytochemicals. The high quantity of alkaloids (0.153.8±45.8 mg/g), saponins (211±70.40 mg/g) were detected in stem, flavonoids (144±3.16 mg/g) in leaf, terpenoids (34.0±4.1 mg/g) in bark. The phenol (9.49±0.0002mg/g) and the tannin (79.03±0.019 mg/g) present in the bark was examined by using standard gallic acid and ta...
Recently, development of reliable experimental protocols for synthesis of metal nanoparticles wit... more Recently, development of reliable experimental protocols for synthesis of metal nanoparticles with desired morphologies and sizes has become a major focus of researchers. Green synthesis of metal nanoparticles using plant extracts emerged as a nontoxic and eco-friendly method for synthesis of metal nanoparticles. In this study biosynthesis of stable copper nanoparticles were done using aqueous leaf extract of Vitex negundo leaf from 3mm copper sulphate solution. Synthesized nanoparticles were characterized under UV-Vis spectroscopy at the range of 400 nm to 800 nm at varying intervals of time. The peak at 254nm revealed the presence of CuNPs. It was observed that the Vitex negundo leaf extract can reduce copper ions into copper nanoparticles within 10 to 15 min of reaction time. These biosynthesized Cu nanoparticles were characterized with the help of X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and SEM techniques. GC-MS analysis revealed that the leaf extract of Vitex negundo.L,contains steroids, saponins, tannins, phenols, triterpenoids, flavonoids, glycosides, and glycerides. These bioactive principles are found to be responsible for bioreduction during the synthesis of spherical copper nanoparticles. The preparation of nano-structured copper particles using Vitex negundo.L leaf extract thus provides an environmentally friendly option, as compared to currently available chemical and physical methods.
The indigenous plant of Manilkara hexandra belongs to Sapotaceae family. It is also called in tam... more The indigenous plant of Manilkara hexandra belongs to Sapotaceae family. It is also called in tamil " Kanuppalai ". The sacred plant is widely observed in the religious temple of South India because of it consists of latex. The investigation is carried on phytochemical screening analysis, quantitative and yield percentage of leaf, stem, and bark of Manilkara hexandra. The dried powder was screened with petroleum ether, hexane, chloroform, ethyl acetae, ethanol, methanol and double distilled water by cold extraction method. The secondary metabolites were estimated with standard procedures. The yield percentage was calculated. The qualitative report shows the presence of phytochemicals. The high quantity of alkaloids (0.153.8±45.8 mg/g), saponins (211±70.40 mg/g) were detected in stem, flavonoids (144±3.16 mg/g) in leaf, terpenoids (34.0±4.1 mg/g) in bark. The phenol (9.49±0.0002mg/g) and the tannin (79.03±0.019 mg/g) present in the bark was examined by using standard gallic acid and tannic acid respectively. The yield reveals that the amount of stem (27.64 g in %) is rich in ethanol. From the consecutive results, concluded that the rich herbal compounds are utilized for future research on innovative drug delivery system.
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Papers by Dr.S. Irudaya Monisha