Brittany Brown

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Dr.P.Madhava Soma sundaram

Manonmaniam Sundaranar University

Richard Jochelson

University of Manitoba

Dalibor Dolezal

University of Zagreb, Faculty of Education and Rehabilitation Sciences

Kevin Wright

Arizona State University

Lirieka Meintjes-van Der Walt

University of Fort Hare

John Danaher

University of Galway

Diego Calvo Merino

Facultad Adventista de Teología de España

Kevin Walby

University of Winnipeg

telmo viteri

Universidad de Guayaquil

Marisol Mora

Universitat Autònoma de Barcelona

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Development of a cell free virus free beads-based SARS-Cov-2 virus neutralization assay

The Journal of Immunology

Development of a simple, fast, and safe method to detect neutralizing antibody levels to a pathog... more Development of a simple, fast, and safe method to detect neutralizing antibody levels to a pathogen is urgent to help understand the immune status of convalescent patients and the immune responses after vaccine. Here we report the development of a cell-free and virus-free beads-based virus neutralization assay. We focused on the detection of neutralization antibody (nAb) in blood circulation to SARS-Cov-2 spike protein receptor binding domain (RBD). This assay uses histidine tagged recombinant RBD protein to replace living virus and uses streptavidin coated polystyrene beads as a carrier of human angiotensin-converting enzyme 2 (hACE2). This bead serves as a surrogate for target cells. Neutralization was detected by the decrease or absence of fluorescence signal associated with RBD. The assay was compared to a cell-based neutralization assay and ELISA, and these results are reported. The bead-based assay is simple, fast, safe and importantly, can truly reflect the immune status of i...

iRock 2.0 and the iR-Processor: an automated immune repertoire library preparation platform for NGS (TECH2P.919)

The Journal of Immunology

Postpartum Migraine Headache Coding in Electronic Health Records of a Large Integrated Health Care System: Validation Study

JMIR Formative Research

Background Migraine is a common neurological disorder characterized by repeated headaches of vary... more Background Migraine is a common neurological disorder characterized by repeated headaches of varying intensity. The prevalence and severity of migraine headaches disproportionally affects women, particularly during the postpartum period. Moreover, migraines during pregnancy have been associated with adverse maternal outcomes, including preeclampsia and postpartum stroke. However, due to the lack of a validated instrument for uniform case ascertainment on postpartum migraine headache, there is uncertainty in the reported prevalence in the literature. Objective The aim of this study was to evaluate the completeness and accuracy of reporting postpartum migraine headache coding in a large integrated health care system’s electronic health records (EHRs) and to compare the coding quality before and after the implementation of the International Classification of Diseases, 10th revision, Clinical Modification (ICD-10-CM) codes and pharmacy records in EHRs. Methods Medical records of 200 del...

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